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. 1974 Jul;141(1):313-5.
doi: 10.1042/bj1410313.

Specificity of anti-nucleoside antibodies

M Z Humayun, T M Jacob

Specificity of anti-nucleoside antibodies

M Z Humayun et al. Biochem J. 1974 Jul.

Abstract

The method of conjugation of a nucleoside or related compound to a carrier protein may have a significant effect on the specificity of the antibodies elicited. It is demonstrated, by means of the membrane-filtration assay, that anti-isopentenyladenosine antibodies produced by the ;periodate procedure' are much more reactive with the periodate-oxidized form of the nucleoside than with the parent compound. In addition, the simplicity and specificity of the assay used suggests its use as a sensitive radioimmunoassay for this multifunctional nucleoside.

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