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. 1969 Mar;17(3):422-6.
doi: 10.1128/am.17.3.422-426.1969.

Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells

P J Baker, P W Stashak, B Prescott

Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells

P J Baker et al. Appl Microbiol. 1969 Mar.

Abstract

A method was described for the sensitization of erythrocytes with purified type-specific pneumococcal polysaccharide antigens using chromium chloride as a coupling agent. Erythrocytes so sensitized can be used in routine passive hemagglutination and hemolysis tests as well as in the technique of localized hemolysis-in-gel for the detection of specific antibody and specific antibody-producing cells, respectively.

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References

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