Potassium ion release and enzyme secretion: adrenergic regulation by alpha- and beta-receptors

Abstract

Epinephrine caused amylase secretion and K(+) release in rat parotid slices. Propranolol, which blocks beta-receptors, inhibited amylase secretion; phentolamine, which blocks alpha-receptors, inhibited K(+) release. Since enzyme secretion was associated with fusion of secretory granules to the cell membrane and K(+) release was associated with vacuole formation, it could be shown that both alpha- and beta-receptors are present in the same exocrine cell. The findings appear to exclude cyclic 3',5'-adenosine monophosphate as an intermediate in the alpha-receptor response.