Some observations on liver cell proliferation in the isolated perfused rat liver

Abstract

Experiments were designed to test the hypothesis that in an isolated perfused system a regenerating liver is able to stimulate a normal liver to increase DNA replication after cross-circulation for one hour. The high degree of purity of DNA extracted by the “phenol” technique is demonstrated and the suitability of this technique for rat livers emphasized. The effect of chemiluminescence on tritiated thymidine is presented.

The results of experiments presented here, together with other evidence, indicate the improbability of a stimulus being able to induce increased DNA synthesis in a normal liver after an exposure of only one hour.