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. 1974 Jul;141(1):159-64.
doi: 10.1042/bj1410159.

Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii

N S Hettiarachchy, I G Jones

Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii

N S Hettiarachchy et al. Biochem J. 1974 Jul.

Abstract

DNA was prepared from isolated mitochondria of Acanthamoeba castellanii and was shown to behave as a single component in density gradients, on ;melting' and on renaturation. From measurements of renaturation kinetics, sedimentation coefficient and electron micrographs the genome size of the mitochondrial DNA was calculated to be about 3.4x10(7) daltons. A small proportion of the preparations could be isolated as relaxed circular molecules of mean contour length 16.2mum.

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References

    1. Biochim Biophys Acta. 1965 Sep 6;108(1):18-29 - PubMed
    1. J Mol Biol. 1967 May 28;26(1):125-9 - PubMed
    1. J Mol Biol. 1968 Feb 14;31(3):349-70 - PubMed
    1. Virology. 1968 Apr;34(4):738-47 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1968;33:435-42 - PubMed