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. 1974 Jul;141(1):159-64.
doi: 10.1042/bj1410159.

Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii

N S Hettiarachchy, I G Jones

Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii

N S Hettiarachchy et al. Biochem J. 1974 Jul.

Abstract

DNA was prepared from isolated mitochondria of Acanthamoeba castellanii and was shown to behave as a single component in density gradients, on ;melting' and on renaturation. From measurements of renaturation kinetics, sedimentation coefficient and electron micrographs the genome size of the mitochondrial DNA was calculated to be about 3.4x10(7) daltons. A small proportion of the preparations could be isolated as relaxed circular molecules of mean contour length 16.2mum.

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