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. 1974 Jul;141(1):51-5.
doi: 10.1042/bj1410051.

The utility of photo-affinity labels as "mapping" reagents. A study of sub-populations of a specific rabbit antibody by using structurally related photo-affinity reagents

R A Smith, J R Knowles

The utility of photo-affinity labels as "mapping" reagents. A study of sub-populations of a specific rabbit antibody by using structurally related photo-affinity reagents

R A Smith et al. Biochem J. 1974 Jul.

Abstract

A specific rabbit antibody against the 4-azido-2-nitrophenyl determinant was photo-labelled by the homologous hapten epsilon-(4-azido-2-nitrophenyl)-l-lysine, and by the close structural isomer epsilon-(5-azido-2-nitrophenyl)-l-lysine. The extents of covalent labelling of the antibody-binding site were assessed by using radioactive haptens and exhaustive displacement dialysis, which leaves the unlabelled sites empty but largely intact. A single photolysis of hapten-antibody complex suffices to label those sites that are capable of being labelled. Although there is considerable overlap among sub-populations of antibody that will bind the two haptens non-covalently, sites that can be covalently labelled by one reagent cannot be labelled by the other.

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References

    1. Biochem J. 1972 Jul;128(3):499-508 - PubMed
    1. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1656-61 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Apr;51:612-8 - PubMed
    1. Biochem J. 1963 Aug;88:228-34 - PubMed
    1. Biochem J. 1968 May;107(6):753-63 - PubMed