Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Jul;141(1):71-8.
doi: 10.1042/bj1410071.

Calcium and the assays of human plasma clotting factor XIII

R D Cooke, J J Holbrook

Calcium and the assays of human plasma clotting factor XIII

R D Cooke et al. Biochem J. 1974 Jul.

Abstract

1. A continuous fluorimetric assay for blood-clotting Factor XIII based on the incorporation of dansylcadaverine into casein was investigated. 2. Hammarsten casein was fractionated to yield the beta-casein, which was dephosphorylated and acetylated to give a substrate which itself did not bind calcium and which was not cross-linked by the activated Factor. 3. The modified beta-casein was used as substrate for a continuous fluorimetric assay and as substrate for the incorporation of radioactive glycine ethylester. 4. A linked fluorimetric assay for the zymogen is described. 5. The K(m) for calcium was redetermined and at 0.2mm was in the physiological range and much lower than the values reported by others using substrates which interact with calcium. 6. The K(m) for casein is about 14mum.

PubMed Disclaimer

References

    1. Biochemistry. 1966 Apr;5(4):1413-25 - PubMed
    1. Biochemistry. 1966 May;5(5):1747-53 - PubMed
    1. Biochem Biophys Res Commun. 1966 Sep 22;24(6):858-66 - PubMed
    1. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 - PubMed
    1. Science. 1968 May 24;160(3830):892-3 - PubMed