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. 1974 Oct;143(1):137-41.
doi: 10.1042/bj1430137.

Conformational changes in the extracellular beta-lactamase I from Bacillus cereus 569/H/9

R B Davies, E P Abraham

Conformational changes in the extracellular beta-lactamase I from Bacillus cereus 569/H/9

R B Davies et al. Biochem J. 1974 Oct.

Abstract

1. The thermal denaturation and precipitation of beta-lactamase I from Bacillus cereus 569/H/9 at 60 degrees C are reversible, a soluble and almost fully active enzyme being obtained after solution of the precipitate in 5m-guanidinium chloride or 8m-urea and subsequent removal of the denaturing agent. 2. Inactivation of beta-lactamase I occurs rapidly between 50 degrees and 55 degrees C and is shown by circular-dichroism spectra to be accompanied by an extensive conformational change. 3. A change to a different conformation occurs in 6m-urea. This change is also reversible; refolding with almost complete recovery of enzymic activity occurs within 5min of dilution of the denaturing agent. 4. Inactivation of beta-lactamase I at pH3.0 and 11.0 is also associated with conformational changes, since a proportion of the lost activity is recovered within 5min of adjustment of the pH to 7.0.

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References

    1. J Biol Chem. 1970 May 10;245(9):2205-12 - PubMed
    1. J Biol Chem. 1970 Jul 25;245(14):3556-60 - PubMed
    1. Biochem J. 1970 Jul;118(3):457-65 - PubMed
    1. Biochim Biophys Acta. 1971 Jun 29;236(3):619-27 - PubMed
    1. Biochem J. 1962 Apr;83:52-63 - PubMed