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. 1974 Oct;143(1):67-72.
doi: 10.1042/bj1430067.

Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis

L A Burgoyne, D R Hewish, J Mobbs

Mammalian chromatin substructure studies with the calcium-magnesium endonuclease and two-dimensional polyacrylamide-gel electrophoresis

L A Burgoyne et al. Biochem J. 1974 Oct.

Abstract

The basic regularity of chromatin substructure that has been reported in rat liver chromatin (Hewish & Burgoyne, 1973b) was also detected in mouse chromatin. The regular series of DNA fragments produced by the action of Ca-Mg endonuclease on rat chromatin were studied further. The smallest single-stranded class has a molecular weight of approx. 45000-63000 and the smallest double-stranded class has a molecular weight of approx. 120000-150000. Studies of the substructure of the DNA fragments produced by the Ca-Mg endonuclease have shown that the regular series of double-stranded fragments have regular series of single-stranded fragments within them. It was concluded that the regular series of double-stranded fragments was probably a consequence of the regular series of single-stranded fragments. Digestion time-courses are presented for mouse and rat nuclear DNA.

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References

    1. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2115-9 - PubMed
    1. Biochemistry. 1972 Mar 28;11(7):1242-50 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3243-6 - PubMed
    1. Biochim Biophys Acta. 1972 Oct 27;281(3):453-62 - PubMed
    1. Anal Biochem. 1972 Dec;50(2):519-28 - PubMed