doi: 10.1007/BF01964390.
The morphology of cytoplasts and karyoplasts produced by cytochalasin D
- PMID: 446642
- DOI: 10.1007/BF01964390
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The morphology of cytoplasts and karyoplasts produced by cytochalasin D
Experientia.
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Abstract
The denucleation of L cells and human lymphocytes in suspension was carried out by incubation with cytochalasin D25 and 50 microgram/ml and with ultracentrifugation over a discontinuous Ficoll gradient. Ultrastructural examination of the separate layers confirmed the presence of the anucleate cytoplasm (cytoplasts) with intact membrane and the separated nuclei (karyoplasts).
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