In vitro production of IgE and IgG by sensitized human peripheral lymphocytes

Abstract

Antigenic stimulation of peripheral lymphocytes was made in two different groups of atopic patients, testing their capabilities to produce IgE and IgG in vitro. For this purpose, Marbrook's technique was used in the cultures. The immunoglobulins produced were measured in the supernatants by a sensitive double-antibody method. Results demonstrate that it is possible to produce enough IgE and IgG to be detected. Some important parameters had to be considered: appropriate selection of the patients and the antigens used in the antigenic stimulation, as well as the number of cells and the duration of the culture.