Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 May;139(2):375-80.
doi: 10.1042/bj1390375.

An investigation by dielectric methods of hydration in myoglobin solutions

E H Grant, B G Mitton, G P South, R J Sheppard

An investigation by dielectric methods of hydration in myoglobin solutions

E H Grant et al. Biochem J. 1974 May.

Abstract

The permittivities of three solutions of sperm-whale myoglobin of different concentrations were measured in the frequency range 300-1300MHz at 20 degrees C by using a coaxial-line technique. These results were combined with those measured previously at frequencies below 10MHz. Two methods are described for calculating the extent of macromolecular hydration from the data. The more reliable method yields results of approx. 0.25g of H(2)O/g of protein, which is in satisfactory agreement with the theoretically calculated value. Agreement with the value found from the rotational motion of the molecule is not so close, which is probably caused by the different meanings that may be ascribed to the term hydration.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1965 Nov 29;109(2):544-50 - PubMed
    1. J Phys Chem. 1968 Dec;72(13):4373-80 - PubMed
    1. Biochem J. 1968 Dec;110(3):419-24 - PubMed
    1. J Phys Chem. 1969 Aug;73(8):2600-10 - PubMed
    1. Biochem J. 1971 May;122(5):691-9 - PubMed