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. 1972 Jan;69(1):115-8.
doi: 10.1073/pnas.69.1.115.

Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR)

D A Usher, E S Erenrich, F Eckstein

Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR)

D A Usher et al. Proc Natl Acad Sci U S A. 1972 Jan.

Abstract

Advantage was taken of the reversibility of the first step of ribonuclease-A action to synthesize the dinucleoside phosphorothioate Up(S)C from the crystalline isomer of uridine 2',3'-cyclic phosphorothioate [Up(S)] and cytidine. Cyclic phosphorothioate was then reformed from Up(S)C by a nonenzymic reaction known to proceed by an in-line mechanism. The geometry of the enzymic reaction was determined to be in-line by a comparison of the Up(S) product with the Up(S) originally used. By the principle of microscopic reversibility, the geometry of the first step in the action of ribonuclease-A is shown to be in-line.

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References

    1. Eur J Biochem. 1967 Mar;1(1):36-45 - PubMed
    1. Nature. 1970 Nov 14;228(5272):663-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1151-8 - PubMed
    1. FEBS Lett. 1968 Dec;2(2):85-86 - PubMed
    1. Science. 1968 Jul 26;161(3839):328-34 - PubMed

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