doi: 10.1073/pnas.69.1.139.
Evidence of gene diminution during the formation of the macronucleus in the protozoan, Stylonychia (DNA density-melting curves-micronuclear DNA-polytene chromosomes)
- PMID: 4500545
- PMCID: PMC427562
- DOI: 10.1073/pnas.69.1.139
Item in Clipboard
Evidence of gene diminution during the formation of the macronucleus in the protozoan, Stylonychia (DNA density-melting curves-micronuclear DNA-polytene chromosomes)
Proc Natl Acad Sci U S A.
1972 Jan.
Abstract
The course of events by which a macronucleus is formed from a micronucleus after conjugation in the ciliated protozoan, Stylonychia, suggests that genetic diminution may occur. This idea is supported by determinations of the density profiles and melting curves for micro- and macronuclear DNAs. Macronuclear DNA consists of a single density component and melts as if it were a single component. Micronuclear DNA consists of four or more density components and melts as if it were a mixture of several DNAs of different base compositions. These data indicate that at least 60% of the micronuclear DNA components are absent from the macronuclear DNA.
Similar articles
-
Micronuclear DNA sequences of Oxytricha fallax homologous to the macronuclear inverted terminal repeat.Nucleic Acids Res. 1982 May 11;10(9):2911-24. doi: 10.1093/nar/10.9.2911. Nucleic Acids Res. 1982. PMID: 7099969 Free PMC article.
-
DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha.Cell. 1976 Jan;7(1):67-74. doi: 10.1016/0092-8674(76)90256-7. Cell. 1976. PMID: 820431
-
The formation of new nucleoli during macronuclear development of the hypotrichous ciliate Stylonychia lemnae visualized by in situ hybridization.Chromosome Res. 1997 Aug;5(5):333-5. doi: 10.1023/B:CHRO.0000038764.83094.aa. Chromosome Res. 1997. PMID: 9292238
-
Polytene chromosomes of Oxytricha: biochemical and morphological changes during macronuclear development in a ciliated protozoan.Chromosoma. 1976 Jan 27;54(1):1-13. doi: 10.1007/BF00331828. Chromosoma. 1976. PMID: 813980
-
De novo cytosine methylation in the differentiating macronucleus of the stichotrichous ciliate Stylonychia lemnae.Nucleic Acids Res. 2003 Mar 1;31(5):1387-91. doi: 10.1093/nar/gkg233. Nucleic Acids Res. 2003. PMID: 12595545 Free PMC article.
Cited by
-
Macronuclear DNA of the hypotrichous ciliate Oxytricha fallax.Proc Natl Acad Sci U S A. 1978 Oct;75(10):4992-6. doi: 10.1073/pnas.75.10.4992. Proc Natl Acad Sci U S A. 1978. PMID: 105360 Free PMC article.
-
Studies on Macronuclear DNA from Paramecium aurelia.Chromosoma. 1975 Dec 10;53(3):191-208. doi: 10.1007/BF00329171. Chromosoma. 1975. PMID: 1201690
-
Development of the macronuclear anlage in the ciliate Chilodonella uncinate. I. Morphological and cytophotometric studies on the evolution of DNA.Chromosoma. 1974;48(3):225-38. doi: 10.1007/BF00326506. Chromosoma. 1974. PMID: 4217265 No abstract available.
-
Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates.Proc Natl Acad Sci U S A. 1975 Feb;72(2):678-82. doi: 10.1073/pnas.72.2.678. Proc Natl Acad Sci U S A. 1975. PMID: 804696 Free PMC article.
-
Micronuclear DNA sequences of Oxytricha fallax homologous to the macronuclear inverted terminal repeat.Nucleic Acids Res. 1982 May 11;10(9):2911-24. doi: 10.1093/nar/10.9.2911. Nucleic Acids Res. 1982. PMID: 7099969 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
