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. 1972 Mar;69(3):681-5.
doi: 10.1073/pnas.69.3.681.

Characterization of messenger ribonucleoprotein and messenger RNA from KB cells

A Kumar, U Lindberg

Characterization of messenger ribonucleoprotein and messenger RNA from KB cells

A Kumar et al. Proc Natl Acad Sci U S A. 1972 Mar.

Abstract

Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 x 10(6).

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References

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