doi: 10.1073/pnas.69.6.1375.
Initiation of protein synthesis in HeLa cells
- PMID: 4504345
- PMCID: PMC426705
- DOI: 10.1073/pnas.69.6.1375
Item in Clipboard
Initiation of protein synthesis in HeLa cells
Proc Natl Acad Sci U S A.
1972 Jun.
Abstract
Initiation of protein synthesis in HeLa cells has been synchronized by exposure of the cells to fluoride. Double-labeling of such cells for short pulses with [(35)S]methionine and a tritiated amino acid, followed by Edman degradation of the puromycin-released nascent peptides, has shown that the percent of N-terminal methionine incorporated compared to total incorporation is significantly higher than the value obtained with any of the other amino acids tested. The results suggest that the bulk of the nascent proteins synthesized in vivo by HeLa cells are initiated with methionine.
Similar articles
-
Stimulation of polypeptide initiation in vitro after protein synthesis inhibition in vivo in HeLa cells.Proc Natl Acad Sci U S A. 1973 Sep;70(9):2678-82. doi: 10.1073/pnas.70.9.2678. Proc Natl Acad Sci U S A. 1973. PMID: 4517679 Free PMC article.
-
Initiation of protein synthesis in vivo in poliovirus-infected HeLa cells.Arch Biochem Biophys. 1973 Jan;154(1):431-7. doi: 10.1016/0003-9861(73)90076-3. Arch Biochem Biophys. 1973. PMID: 4347688 No abstract available.
-
Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids.Proc Natl Acad Sci U S A. 1971 Sep;68(9):2057-61. doi: 10.1073/pnas.68.9.2057. Proc Natl Acad Sci U S A. 1971. PMID: 5289364 Free PMC article.
-
Protein biosynthesis.Annu Rev Biochem. 1971;40:409-48. doi: 10.1146/annurev.bi.40.070171.002205. Annu Rev Biochem. 1971. PMID: 4941237 Review. No abstract available.
-
The in vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis.Methods Mol Biol. 1994;32:67-72. doi: 10.1385/0-89603-268-X:67. Methods Mol Biol. 1994. PMID: 7951750 Review. No abstract available.
Cited by
-
Model studies of enzymatic NH2-terminal acetylation of porteins with des-Nalpha1-acetyl-alpha-melanotropin as a substrate.Proc Natl Acad Sci U S A. 1976 Sep;73(9):3010-4. doi: 10.1073/pnas.73.9.3010. Proc Natl Acad Sci U S A. 1976. PMID: 1067599 Free PMC article.
-
The presence of N-terminal methionine on nascent protein of rat liver and rabbit reticulocytes and its cleavage during polypeptide-chain elongation.Biochem J. 1973 Aug;134(4):969-83. doi: 10.1042/bj1340969. Biochem J. 1973. PMID: 4762764 Free PMC article.
-
Identification of N-terminal methionine in the precursor of immunoglobulin light chain. Initiation of translation of messenger ribonucleic acid in plants and animals.Biochem J. 1976 Mar 1;153(3):543-50. doi: 10.1042/bj1530543. Biochem J. 1976. PMID: 821467 Free PMC article.
-
Effect of poliovirus double-stranded RNA on viral and host-cell protein synthesis.Proc Natl Acad Sci U S A. 1974 Jun;71(6):2440-4. doi: 10.1073/pnas.71.6.2440. Proc Natl Acad Sci U S A. 1974. PMID: 4366768 Free PMC article.
-
Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2.Proc Natl Acad Sci U S A. 1974 Aug;71(8):3129-33. doi: 10.1073/pnas.71.8.3129. Proc Natl Acad Sci U S A. 1974. PMID: 4370520 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
