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. 1972 Jun;69(6):1425-8.
doi: 10.1073/pnas.69.6.1425.

Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes

M Jacobs-Lorena, C Baglioni

Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes

M Jacobs-Lorena et al. Proc Natl Acad Sci U S A. 1972 Jun.

Abstract

10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total alpha chain mRNA is present in the postribosomal supernatant, whereas beta chain mRNA is almost exclusively associated with polyribosomes.

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