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. 1972 Sep;69(9):2556-60.
doi: 10.1073/pnas.69.9.2556.

Antigen-binding specificity of isolated cell-surface immunoglobulin from thymus cells activated to histocompatibility antigens

R E Cone, J Sprent, J J Marchalonis

Antigen-binding specificity of isolated cell-surface immunoglobulin from thymus cells activated to histocompatibility antigens

R E Cone et al. Proc Natl Acad Sci U S A. 1972 Sep.

Abstract

Lactoperoxidase-catalyzed radioiodination of cell-surface proteins was used in the isolation of cell-surface immunoglobulin from thymus-derived thoracic duct lymphocytes activated to histocompatibility-2 antigens. Immunoglobulin was identified by specific precipitation with antiserum to mouse immunoglobulin. Polyacrylamide gel electrophoresis of reduced and alkylated precipitates showed that the immunoglobulin molecules possessed mu-type heavy chains and light chains. Cell-surface immunoglobulin isolated from thymus-derived cells activated to histocompatibility-2 antigens possessed binding specificity for the activating antigens.

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