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. 1972 Sep;69(9):2599-603.
doi: 10.1073/pnas.69.9.2599.

An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses

B I Gerwin, J B Milstien

An oligonucleotide affinity column for RNA-dependent DNA polymerase from RNA tumor viruses

B I Gerwin et al. Proc Natl Acad Sci U S A. 1972 Sep.

Abstract

Columns of (dT)(12-18)-cellulose provide a one-step enrichment procedure for RNA-dependent DNA polymerase. The enzyme of the virus from RD-114 cells, as well as that from Rauscher murine leukemia virus, have been purified in this way. The preference of viral as compared to cellular DNA polymerases for (dT)(12-18) as a primer is reflected in the fact that the DNA polymerases of uninfected cells do not bind to this column. Viral enzymes have been purified and identified from crude cellular extracts.

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References

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