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. 1979 Apr;6(4):1683-94.
doi: 10.1093/nar/6.4.1683.

Conformational changes induced in DNA by in vitro reaction with N-hydroxy-N-2-aminofluorene

M Spodheim-Maurizot, G Saint-Ruf, M Leng
Free PMC article

Conformational changes induced in DNA by in vitro reaction with N-hydroxy-N-2-aminofluorene

M Spodheim-Maurizot et al. Nucleic Acids Res. 1979 Apr.
Free PMC article

Abstract

The conformation of DNA modified in vitro by the covalent binding of N-OH-AF was investigated by ultraviolet absorbance, circular dichroism and by radioimmunoassay using specific antibodies against Guo-AAF and nDNA-AAF. The results obtained by both physico-chemical and immunological methods are in agreement with a model involving destabilized regions in the double helical DNA around the carcinogen molecule in which, however, the -AF residues are stacked to the adjacent nucleotides. The RIA results show that the -AF residues are less accessible to antibodies in native than in denatured DNA-AF and thus suggest -AF residues partially buried in the interior of the DNA helix. The present model is compared to the one existing for DNA modified by reaction with N-AcO-AAF (DNA-AAF) (1,2).

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