doi: 10.1073/pnas.69.10.2802.
Formation and decay of prelumirhodopsin at room temperatures
- PMID: 4507603
- PMCID: PMC389648
- DOI: 10.1073/pnas.69.10.2802
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Formation and decay of prelumirhodopsin at room temperatures
Proc Natl Acad Sci U S A.
1972 Oct.
Abstract
We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.
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