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. 1972 Oct;69(10):2802-6.
doi: 10.1073/pnas.69.10.2802.

Formation and decay of prelumirhodopsin at room temperatures

G E Busch, M L Applebury, A A Lamola, P M Rentzepis

Formation and decay of prelumirhodopsin at room temperatures

G E Busch et al. Proc Natl Acad Sci U S A. 1972 Oct.

Abstract

We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.

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References

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