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. 1972 Nov;69(11):3243-6.
doi: 10.1073/pnas.69.11.3243.

Blocking by histones of accessibility to DNA in chromatin: addition of histones

A E Mirsky, B Silverman, N C Panda

Blocking by histones of accessibility to DNA in chromatin: addition of histones

A E Mirsky et al. Proc Natl Acad Sci U S A. 1972 Nov.

Abstract

The blocking effect of histones on accessibility of DNA to DNase has been studied by addition of various histones to chromatin in intact or histone-depleted thymus nuclei (those from which all lysine-rich histone was removed and those from which much of the arginine-rich histone was removed) and to deoxyribonucleoprotein (chromatin) extracted from nuclei. In each case lysine-rich histone, weight for weight, blocks accessibility more effectively than does arginine-rich histone. Fractions of the arginine-rich histone differ considerably from each other in their blocking efficacy. Phosphorylation of lysine-rich histone tempers its blocking effectiveness.

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