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. 1972 Nov;69(11):3423-7.
doi: 10.1073/pnas.69.11.3423.

Transfection mediated by Mycoplasmatales viral DNA

A Liss, J Maniloff

Transfection mediated by Mycoplasmatales viral DNA

A Liss et al. Proc Natl Acad Sci U S A. 1972 Nov.

Abstract

DNA isolated from Mycoplasmatales viruses MVL51 and MVGs51 was infectious when mixed with Acholeplasma laidlawii BN1-Na1(R) cells. Infectivity was destroyed by deoxyribonuclease but not by ribonuclease, Pronase, or specific antiserum to the virus. Host mycoplasma cells were only competent for transfection during late-log growth phase. The rates of the establishment of DNase insensitivity of viral DNA transfectants were similar to those of bacteriophage systems. The dose-response curve for transfection suggested that an average of six molecules of DNA must interact with a cell in order to produce one infectious center. Mycoplasmatales virus DNA exhibited a low efficiency of infection; one infectious center required 4 x 10(5) virus equivalents of DNA.

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