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. 1972 Dec;69(12):3732-6.
doi: 10.1073/pnas.69.12.3732.

Hapten-conjugated antibodies and visual markers used to label cell-surface antigens for electron microscopy: an approach to double labeling

M E Lamm, G C Koo, C W Stackpole, U Hämmerling

Hapten-conjugated antibodies and visual markers used to label cell-surface antigens for electron microscopy: an approach to double labeling

M E Lamm et al. Proc Natl Acad Sci U S A. 1972 Dec.

Abstract

Haptens can be coupled to mouse immunoglobulin with retention of antibody specificity. Such hapten-coupled alloantibody, when bound to cells, can be bridged to an electron microscopic marker in two ways: (i) by a hybrid antibody, i.e., anti-hapten:anti-marker, and (ii) by untreated bivalent anti-hapten antibody if the marker is also haptenized. Through the use of immunologically non-crossreactive haptens and markers it should be possible to localize multiple, different antigens on the surface of an individual cell. Double labeling has been achieved with the first method.

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