Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii
- PMID: 4517675
- PMCID: PMC427073
- DOI: 10.1073/pnas.70.9.2642
Nucleotide sequence of HS-beta satellite DNA from kangaroo rat Dipodomys ordii
Abstract
The sequence of the highly repetitive satellite HS-beta DNA fraction from kangaroo rat Dipodomys ordii was determined independently by RNA and DNA sequencing techniques. A basic iterated sequence of 10 nucleotides with several mutational variations was found. Base-composition data are consistent with the proposed sequence and revealed a high content of 5-methylcytosine. DNA and RNA sequencing techniques used gave identical results, showing that the fidelity of synthesis of riboguanidine-substituted DNA under our conditions is adequate for nucleotide sequence studies.
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