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. 1974 Jan;71(1):26-9.
doi: 10.1073/pnas.71.1.26.

Molecular complementarity of yeast glycoprotein mating factors

M Crandall, L M Lawrence, R M Saunders

Molecular complementarity of yeast glycoprotein mating factors

M Crandall et al. Proc Natl Acad Sci U S A. 1974 Jan.

Abstract

Cell fusion between opposite mating types 5 and 21 of the yeast Hansenula wingei is initiated by a strong sexual agglutination reaction. The mating factors responsible for the specificity of cellular recognition are complementary glycoproteins which form a physical complex in vitro. The complex is assayed by recovery of agglutination activity of the multivalent 5-factor after the univalent 21-factor has been inactivated by treatment of the complex with alkali. The 5-factor.21-factor complex, purified on Sepharose 6B, is large (several million daltons) and heterogeneous. The three peaks of 5-factor activity contain a number of combining sites proportional to molecular size.

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References

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