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. 1973 Nov;70(11):3145-9.
doi: 10.1073/pnas.70.11.3145.

Altered enzymes in drug-resistant variants of mammalian tissue culture cells

J D Sharp, N E Capecchi, M R Capecchi

Altered enzymes in drug-resistant variants of mammalian tissue culture cells

J D Sharp et al. Proc Natl Acad Sci U S A. 1973 Nov.

Abstract

Two selective procedures are compared in an effort to isolate variants of mouse L cells containing structural gene mutations. Among the resulting variant cloned cell lines are found two types of alterations in the enzyme hypoxanthine phosphoribosyl transferase (EC 2.4.2.8) (1): enzyme with altered kinetic constants causing in vivo and in vitro resistance to 8-azaguanine; and (2) enzyme with altered heat sensitivity in vitro. These results support the view that tissue culture cell variants can arise from structural gene mutations.

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References

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