Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Nov;70(11):3260-4.
doi: 10.1073/pnas.70.11.3260.

Application of fingerprinting techniques to iodinated nucleic acids

H D Robertson, E Dickson, P Model, W Prensky

Application of fingerprinting techniques to iodinated nucleic acids

H D Robertson et al. Proc Natl Acad Sci U S A. 1973 Nov.

Abstract

Several techniques of RNA and DNA finger-printing and determination of sequence have been applied to nucleic acids labeled with (125)I. Fingerprints of human 5S RNA and bacteriophage f2 RNA resemble those of their noniodinated counterparts both in complexity and in specific pattern. Iodination as used here is thus a general labeling procedure, and appears principally to label cytidine residues. This iodination method shows little sensitivity to potential structure in single-stranded RNA molecules, yields stable oligonucleotide products in a reproducible manner, and does not change the specificity of several ribonucleases and deoxyribonucleases.

PubMed Disclaimer

References

    1. J Mol Biol. 1965 Sep;13(2):373-98 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11 - PubMed
    1. J Mol Biol. 1967 Oct 14;29(1):27-43 - PubMed
    1. Science. 1967 Dec 29;158(3809):1695-9 - PubMed
    1. Nature. 1969 Sep 6;223(5210):1009-14 - PubMed

MeSH terms

LinkOut - more resources