Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Aug;70(8):2321-5.
doi: 10.1073/pnas.70.8.2321.

Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon

H S Tager, D F Steiner

Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon

H S Tager et al. Proc Natl Acad Sci U S A. 1973 Aug.

Abstract

The heterogeneity of crystalline bovine (ox)/porcine glucagon has been examined by gel filtration and ion-exchange chromatography. A strongly basic peptide that reacted well with antibodies to bovine/porcine glucagon was isolated and its primary structure was determined. The amino-acid sequence of the NH(2)-terminal 29 residues of the 4500-dalton peptide is identical with that of intact bovine or porcine glucagon. The remaining eight residues at its COOH-terminus are Lys-Arg-Asn-Asn-Lys-Asn-Ile-Ala. Small amounts of other glucagon-immunoreactive peptides having molecular weights ranging from 3700 to 9000 were also detected in crystals of bovine/porcine glucagon. We propose that the 37-residue peptide is a fragment of bovine or porcine proglucagon.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1972 Mar;69(3):643-7 - PubMed
    1. J Biol Chem. 1971 May 10;246(9):2822-7 - PubMed
    1. Can J Biochem. 1967 Jul;45(7):1163-74 - PubMed
    1. Biochem Biophys Res Commun. 1971 Oct 15;45(2):387-95 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1521-5 - PubMed

LinkOut - more resources