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. 1974 Aug;71(8):3054-8.
doi: 10.1073/pnas.71.8.3054.

A DNA-protein complex involved in bacteriophage phi chi 174 particle formation

P J Weisbeek, R L Sinsheimer

A DNA-protein complex involved in bacteriophage phi chi 174 particle formation

P J Weisbeek et al. Proc Natl Acad Sci U S A. 1974 Aug.

Abstract

A varphiX-specific DNA-protein complex has been isolated from varphiX-infected cells. This complex contains infective circular single-stranded DNA, the proteins of the genes F, G, H, and J in the same proportions as in the phage particle, and, in addition, the gene D-protein. The D-protein makes up 12-22% of the protein part of the complex. The sedimentation value of the complex is about 140 S. In vitro, the complex can be converted to the normal 114S phage particle (with a concomitant loss of its D-protein) or to an uninfective 70S particle and a small amount of free single-stranded DNA. The fast sedimenting particle is not associated with membranes.

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References

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