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. 1974 Aug;71(8):3204-7.
doi: 10.1073/pnas.71.8.3204.

Methylated messenger RNA synthesis in vitro by purified reovirus

A J Shatkin

Methylated messenger RNA synthesis in vitro by purified reovirus

A J Shatkin. Proc Natl Acad Sci U S A. 1974 Aug.

Abstract

Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.

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References

    1. Nucleic Acids Res. 1974 Jun;1(6):809-22 - PubMed
    1. J Virol. 1968 Oct;2(10):986-91 - PubMed
    1. Biochem Biophys Res Commun. 1968 Dec 30;33(6):895-901 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 - PubMed
    1. Virology. 1969 Dec;39(4):791-810 - PubMed

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