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. 1974 Aug;71(8):3143-6.
doi: 10.1073/pnas.71.8.3143.

Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes

G Huez, G Marbaix, E Hubert, M Leclercq, U Nudel, H Soreq, R Salomon, B Lebleu, M Revel, U Z Littauer

Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes

G Huez et al. Proc Natl Acad Sci U S A. 1974 Aug.

Abstract

The translations of native messenger RNA for rabbit globin and that of poly(A)-free globin messenger RNA have been compared after injection into Xenopus oocytes. The initial rate of translation of poly(A)-free mRNA is close to that found with intact mRNA. However, at longer incubation periods, the rate of globin synthesis with poly(A)-free mRNA is considerably lower than with native mRNA. Similar differences in the template activity of the two mRNA preparations were found with a cell-free extract of Krebs II ascites tumor. It is concluded that the presence of the 3' poly(A)-rich sequence in mRNA is required to ensure high functional stability.

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References

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