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. 1979;45(3):437-50.
doi: 10.1007/BF00443282.

Production, purification and properties of a Pichia kluyveri killer toxin

E J Middelbeek, J M Hermans, C Stumm

Production, purification and properties of a Pichia kluyveri killer toxin

E J Middelbeek et al. Antonie Van Leeuwenhoek. 1979.

Abstract

Production of the killer toxin of Pichia kluyveri 1002 was stimulated in the presence of yeast extract. In a minimal medium production was optimal at pH 3.8-4.0 and 22--25 degrees C. Addition of gelatin and nonionic detergents, like Brij-58 (polyoxyethylene 20 cetyl ether) and Triton-X-100, to this medium enhanced production significantly. The killer toxin was purified 140-fold by use of a stepwise ethanol precipitation and butyl Sepharose column chromatography. The purified killer toxin, which still contained some carbohydrates, appeared to be glycoprotein with a mol wt of about 19 000 and an isoelectric point of 4.3. It was stable between pH 2.5 and 4.7 and up to 40 degrees C.

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References

    1. J Bacteriol. 1972 Oct;112(1):235-42 - PubMed
    1. Nature. 1973 Sep 14;245(5420):81-6 - PubMed
    1. Bacteriol Rev. 1976 Sep;40(3):757-73 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Gen Microbiol. 1974 Apr;81(2):285-9 - PubMed