A limited number of globin genes in human DNA
- PMID: 4530276
- PMCID: PMC434307
- DOI: 10.1073/pnas.71.10.3966
A limited number of globin genes in human DNA
Abstract
The number of globin genes in human cells was determined by hybridizing DNA from human spleens to (3)H-labeled DNA complementary to human globin mRNA. Assuming the rates of reannealing of complementary DNA and cellular DNA are similar, the extent of hybridization of complementary DNA at various ratios of cellular DNA to complementary DNA indicate that there are fewer than 10 globin gene copies per haploid human genome. An alternative analysis of the data, which introduces no assumptions concerning the relative rates of reaction of complementary DNA and cellular DNA, indicates fewer than 20 globin gene copies are present. DNA isolated from the spleen of a patient with beta(+) thalassemia contained a number of globin gene copies similar to that of normal DNA.
Similar articles
-
Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA.J Clin Invest. 1976 Dec;58(6):1475-81. doi: 10.1172/JCI108604. J Clin Invest. 1976. PMID: 993355 Free PMC article.
-
Relative numbers of human globin genes assayed with purified alpha and beta complementary human DNA.Proc Natl Acad Sci U S A. 1975 Apr;72(4):1550-4. doi: 10.1073/pnas.72.4.1550. Proc Natl Acad Sci U S A. 1975. PMID: 1055426 Free PMC article.
-
Human globin gene analysis for a patient with beta-o/delta beta-thalassemia.Proc Natl Acad Sci U S A. 1975 Jun;72(6):2294-9. doi: 10.1073/pnas.72.6.2294. Proc Natl Acad Sci U S A. 1975. PMID: 49057 Free PMC article.
-
[Molecular causes of thalassemia. I. Identification of globin mRNA in homozygous beta-thalassemia using complementary human globin DNA].Mol Biol (Mosk). 1978 Jul-Aug;12(4):787-94. Mol Biol (Mosk). 1978. PMID: 683189 Russian. No abstract available.
-
Direct measurement of the number of globin genes.Br Med Bull. 1976 Sep;32(3):246-50. doi: 10.1093/oxfordjournals.bmb.a071370. Br Med Bull. 1976. PMID: 788833 Review. No abstract available.
Cited by
-
Changes in restricted human cellular DNA fragments containing globin gene sequences in thalassemias and related disorders.Proc Natl Acad Sci U S A. 1978 Mar;75(3):1222-6. doi: 10.1073/pnas.75.3.1222. Proc Natl Acad Sci U S A. 1978. PMID: 274714 Free PMC article.
-
Study of markers of human erythroid differentiation in hybrid cells.In Vitro. 1976 Nov;12(11):734-42. doi: 10.1007/BF02835448. In Vitro. 1976. PMID: 1071439
-
Human placental lactogen mRNA and its structural genes during pregnancy: quantitation with a complementary DNA.Proc Natl Acad Sci U S A. 1977 Mar;74(3):1024-7. doi: 10.1073/pnas.74.3.1024. Proc Natl Acad Sci U S A. 1977. PMID: 66681 Free PMC article.
-
Quantitation of human gamma globin genes and gamma globin mRNA with purified gamma globin complementary DNA.J Clin Invest. 1976 Dec;58(6):1475-81. doi: 10.1172/JCI108604. J Clin Invest. 1976. PMID: 993355 Free PMC article.
-
Relative numbers of human globin genes assayed with purified alpha and beta complementary human DNA.Proc Natl Acad Sci U S A. 1975 Apr;72(4):1550-4. doi: 10.1073/pnas.72.4.1550. Proc Natl Acad Sci U S A. 1975. PMID: 1055426 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
