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. 1974 Dec;71(12):4768-72.
doi: 10.1073/pnas.71.12.4768.

Purified acetylcholine receptor: its reconstitution to a chemically excitable membrane

D M Michaelson, M A Raftery

Purified acetylcholine receptor: its reconstitution to a chemically excitable membrane

D M Michaelson et al. Proc Natl Acad Sci U S A. 1974 Dec.

Abstract

Association of purified acetylcholine receptor from Torpedo californica electroplax with lipids from the same organism results in a vesicular membrane system in which the receptor protein is oriented so that all neurotoxin binding sites appear to be on the outer surface. The reconstituted system is chemically excitable by acetylcholine and carbamylcholine, as measured by (22)Na(+) efflux. This excitability is specifically blocked by the antagonist alpha-bungarotoxin. These results demonstrate that the purified receptor macromolecule contains not only the specific neurotransmitter binding site but also the molecular elements necessary for ion translocation in order to effect postsynaptic depolarization.

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References

    1. Biochim Biophys Acta. 1972 Apr 18;265(2):241-96 - PubMed
    1. Can J Biochem Physiol. 1959 Aug;37(8):911-7 - PubMed
    1. J Biol Chem. 1972 Dec 25;247(24):8198-200 - PubMed
    1. FEBS Lett. 1972 Nov 15;28(1):107-11 - PubMed
    1. Arch Biochem Biophys. 1973 Jan;154(1):270-6 - PubMed

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