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. 1972 Jul;60(1):1-19.
doi: 10.1085/jgp.60.1.1.

Voltage clamp experiments of single muscle fibers of Rana pipiens

L E Moore

Voltage clamp experiments of single muscle fibers of Rana pipiens

L E Moore. J Gen Physiol. 1972 Jul.

Abstract

A voltage clamp for single muscle fibers has been developed. Stability of the system was achieved when an artificial node was created by enclosing a single muscle fiber in a petroleum jelly seal which served as an analogue of the myelin sheath. Typical voltage clamp records were obtained with large inward transient currents followed by a delayed rectification of the outward currents. These currents looked qualitatively similar when the transverse tubular system was destroyed. Errors in current measurement, especially those due to anomalous rectification, are discussed.

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