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. 1979 Jun 13;554(1):259-63.
doi: 10.1016/0005-2736(79)90023-3.

A rapid method for the isolation of kidney brush border membranes

P Malathi, H Preiser, P Fairclough, P Mallett, R K Crane

A rapid method for the isolation of kidney brush border membranes

P Malathi et al. Biochim Biophys Acta. .

Abstract

A simple rapid method for the preparation of purified brush border membranes from rabbit kidney proximal tubules is described. The method is based on hypotonic lysis, Ca2+ aggregation of contaminants and differential centrifugation. In contrast to most other published methods, the brush border membranes are free of contamination by basolateral membranes.

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