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. 1979 Jun 12;585(2):315-9.
doi: 10.1016/0304-4165(79)90031-x.

Interaction of metallochromic indicators with calcium sequestering organelles

Interaction of metallochromic indicators with calcium sequestering organelles

S T Ohnishi. Biochim Biophys Acta. .

Abstract

Examples are presented of the interaction between cell organelles and metallochromic indicators used in the measurement of ionized Ca2+. Sarcoplasmic reticulum was found to sequester murexide type indicators along with Ca2+ in the presence of ATP, but not to sequester arsenazo III and antipyrylazo III. The presence of a permeable anion suppresses the sequestration of murexide type indicators by the sarcoplasmic reticulum. In the presence of ruthenium red, both rat liver and beef heart mitochondria release sequestered Ca2+ with arsenazo III, but not with murexide.

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