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. 1972 Feb;23(2):368-71.
doi: 10.1128/am.23.2.368-371.1972.

Simple method for the preparation of streptococcal nucleases

S C Marker, E D Gray

Simple method for the preparation of streptococcal nucleases

S C Marker et al. Appl Microbiol. 1972 Feb.

Abstract

A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparation of sufficient homogeneous nuclease B for a large number of anti-nuclease B titrations.

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References

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