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. 1972 Apr;110(1):56-65.
doi: 10.1128/jb.110.1.56-65.1972.

Induction of the ara operon of Escherichia coli B-r

M E Doyle, C Brown, R W Hogg, R B Helling

Induction of the ara operon of Escherichia coli B-r

M E Doyle et al. J Bacteriol. 1972 Apr.

Abstract

The inducer specificity and kinetics of induction of the ara operon were examined in Escherichia coli B/r. A difference in the kinetics of induction was found between our B/r strains and the K-12 strain previously described by Schleif. The roles of active transport and metabolism of inducer, and of cell density, in induction were studied. d-Fucose and beta-methyl-l-arabinoside were competitive inhibitors of induction. No inducer of the wild-type strain other than l-arabinose was found. However, a procedure for selecting mutants with altered inducer affinity or specificity was developed. The properties of one such mutant (inducible by d-fucose) are described.

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References

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