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. 1972 Jun 1;135(6):1410-5.
doi: 10.1084/jem.135.6.1410.

Classification of thymus-derived and marrow-derived lymphocytes by demonstration of their antigen-binding characteristics

J S Haskill, B E Elliott, R Kerbel, M A Axelrad, D Eidinger

Classification of thymus-derived and marrow-derived lymphocytes by demonstration of their antigen-binding characteristics

J S Haskill et al. J Exp Med. .

Abstract

Antigen-binding cells of T and B origin can readily be determined by quantitating the number of sheep erythrocytes per rosette after glutaraldehyde fixation. The T(1) and T(2) populations have low antigen-binding properties and are very unstable without fixation. The B(1) and B(2) populations are stable and correlate with precursor and secretory cells. Fixation of rosettes permits a sensitive test for studying differentiation of T and B cells.

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References

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