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. 1972 Jun;110(3):831-42.
doi: 10.1128/jb.110.3.831-842.1972.

Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli

M Achtman, N Willetts, A J Clark

Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli

M Achtman et al. J Bacteriol. 1972 Jun.

Abstract

A series of 102 transfer-deficient (tra(-)) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F'/F') heterozygote cells in transient populations, and for measuring complementation between different Flac tra(-) mutants. These techniques were used to define nine tra cistrons and to assign most of the tra(-) mutations to one or other of these.

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