doi: 10.1073/pnas.69.7.1786.
In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli
- PMID: 4558657
- PMCID: PMC426802
- DOI: 10.1073/pnas.69.7.1786
Item in Clipboard
In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli
Proc Natl Acad Sci U S A.
1972 Jul.
Abstract
Two active enzymes of the tryptophan operon of E. coli, anthranilate synthetase (product of trp E and trp D) and tryptophan synthetase (EC 4.2.1.20) (product of trp B and trp A), have been synthesized in a DNA-dependent preparation for protein synthesis. Anthranilate synthetase and the two components of tryptophan synthetase (tryptophan synthetase A and B) are made in vitro in equimolar amounts, suggesting that regulation of the translation is similar in vivo and in vitro. Kinetics of the synthesis of the two enzymes suggest that transcription and translation proceed in vitro in a correct temporal sequence.
Similar articles
-
Metabolic interlock. The dual function of a folate pathway gene as an extra-operonic gene of tryptophan biosynthesis.J Biol Chem. 1972 Mar 10;247(5):1587-96. J Biol Chem. 1972. PMID: 4622231 No abstract available.
-
Nucleotide sequences from tryptophan messenger RNA of Escherichia coli: the sequence corresponding to the amino-terminal region of the first polypeptide specified by the operon.Proc Natl Acad Sci U S A. 1973 Aug;70(8):2335-9. doi: 10.1073/pnas.70.8.2335. Proc Natl Acad Sci U S A. 1973. PMID: 4599621 Free PMC article.
-
Repression of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) and enzymes of the tryptophan pathway in Escherichia coli K-12.J Bacteriol. 1971 Aug;107(2):406-14. doi: 10.1128/jb.107.2.406-414.1971. J Bacteriol. 1971. PMID: 4939760 Free PMC article.
-
Anthranilate synthetase.Adv Enzymol Relat Areas Mol Biol. 1973;38:1-39. doi: 10.1002/9780470122839.ch1. Adv Enzymol Relat Areas Mol Biol. 1973. PMID: 4275326 Review. No abstract available.
-
Amino acid metabolism.Annu Rev Biochem. 1973;42(0):113-34. doi: 10.1146/annurev.bi.42.070173.000553. Annu Rev Biochem. 1973. PMID: 4151747 Review. No abstract available.
Cited by
-
Expression of the trp operon in phi80trp transducing phages. Orientation of transcription and an artificial high-efficiency promotor in phage lambda h +phi80 pt5-2AB.Mol Gen Genet. 1973 Jan 18;120(1):55-68. doi: 10.1007/BF00332984. Mol Gen Genet. 1973. PMID: 4686211 No abstract available.
-
The specificity of transcription in vitro of the tryptophan operon of Escherichia coli. II. The effect of Rho factor.Mol Gen Genet. 1974;132(4):291-306. doi: 10.1007/BF00268570. Mol Gen Genet. 1974. PMID: 4214990 No abstract available.
-
RNA-directed cell-free synthesis of the galactose enzymes of Escherichia coli.Mol Gen Genet. 1973 Aug 28;124(4):329-44. doi: 10.1007/BF00267662. Mol Gen Genet. 1973. PMID: 4355744 No abstract available.
-
In vitro synthesis of a constitutive enzyme of Escherichia coli, 6-phosphogluconate dehydrogenase.Proc Natl Acad Sci U S A. 1975 Nov;72(11):4381-4. doi: 10.1073/pnas.72.11.4381. Proc Natl Acad Sci U S A. 1975. PMID: 172907 Free PMC article.
-
The effect of the ribosomal protein S1 from Escherichia coli on the synthesis in vitro of bacterial-, DNA phage- and RNA phage proteins.Mol Gen Genet. 1977 May 20;153(1):75-80. doi: 10.1007/BF01035998. Mol Gen Genet. 1977. PMID: 329105
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
