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. 1972 Jul;69(7):1905-10.
doi: 10.1073/pnas.69.7.1905.

Deletion mutants of bacteriophage phiX174

A J Zuccarelli, R M Benbow, R L Sinsheimer

Deletion mutants of bacteriophage phiX174

A J Zuccarelli et al. Proc Natl Acad Sci U S A. 1972 Jul.

Abstract

Mutants of bacteriophage varphiX174 have been isolated that are less dense than wild-type varphiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement varphiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the varphiX genome in the region of cistron E.

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References

    1. Proc Natl Acad Sci U S A. 1969 Oct;64(2):613-7 - PubMed
    1. J Mol Biol. 1971 May 14;57(3):541-53 - PubMed
    1. Science. 1969 Mar 21;163(3873):1343-8 - PubMed
    1. J Mol Biol. 1966 Jul;18(3):429-47 - PubMed
    1. Biochim Biophys Acta. 1969 Oct 22;190(2):460-9 - PubMed

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