doi: 10.1073/pnas.69.7.1905.
Deletion mutants of bacteriophage phiX174
- PMID: 4558663
- PMCID: PMC426829
- DOI: 10.1073/pnas.69.7.1905
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Deletion mutants of bacteriophage phiX174
Proc Natl Acad Sci U S A.
1972 Jul.
Abstract
Mutants of bacteriophage varphiX174 have been isolated that are less dense than wild-type varphiX particles in CsCl. When mutant viral (+) strand DNA and wild-type complementary (-) strand DNA are hybridized, the resulting duplex molecules have single-stranded loops characteristic of wild-type-deletion heteroduplexes. The mutant bacteriophages fail to complement varphiX amber mutants in cistron E. We conclude that the mutant viruses have deleted approximately 7% of the varphiX genome in the region of cistron E.
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