Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Dec;69(12):3602-5.
doi: 10.1073/pnas.69.12.3602.

Release of polypeptide chain initiation factor IF-2 during initiation complex formation

A H Lockwood, P Sarkar, U Maitra

Release of polypeptide chain initiation factor IF-2 during initiation complex formation

A H Lockwood et al. Proc Natl Acad Sci U S A. 1972 Dec.

Abstract

Polypeptide chain initiation factor IF-2 binds to 30S ribosomal subunits. This binding is enhanced by IF-1 and IF-3. During GTP-dependent formation of a 70S initiation complex, IF-2 is released from the ribosome. During 70S initiation complex formation dependent on the methylene analogue of GTP, GMPPCH(2)P, IF-2 is not released, but remains bound to the 70S ribosome. This result suggests that IF-2 release requires GTP hydrolysis. In agreement with this presumed requirement, IF-2 functions catalytically with GTP, but stoichiometrically with GMPPCH(2)P, in bringing about 70S initiation complex formation.

PubMed Disclaimer

References

    1. Arch Biochem Biophys. 1966 Sep 26;116(1):344-51 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Feb;59(2):554-60 - PubMed
    1. Biochem Biophys Res Commun. 1968 Jul 11;32(1):9-14 - PubMed
    1. Nature. 1968 Oct 19;220(5164):244-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1066-70 - PubMed

LinkOut - more resources