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. 1972 Dec;69(12):3756-60.
doi: 10.1073/pnas.69.12.3756.

Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate)

P D Rick, M J Osborn

Isolation of a mutant of Salmonella typhimurium dependent on D-arabinose-5-phosphate for growth and synthesis of 3-deoxy-D-mannoctulosonate (ketodeoxyoctonate)

P D Rick et al. Proc Natl Acad Sci U S A. 1972 Dec.

Abstract

A new type of auxotrophic mutant of Salmonella typhimurium has been isolated that is defective in the synthesis of the 3-deoxy-D-mannooctulosonate (ketodeoxyoctonate) region of the lipopolysaccharide and requires D-arabinose-5-phosphate for growth. Genetic and biochemical evidence indicated that the mutant defect was due to an altered ketodeoxyoctonate-8-phosphate synthetase with an apparent K(m) for D-arabinose-5-phosphate 35-fold higher than that of the parental enzyme. As a result of this enzymatic lesion, the mutant strain was dependent on exogenous D-arabinose-5-phosphate both for growth and for synthesis of a complete lipopolysaccharide.

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References

    1. J Biol Chem. 1966 Jul 10;241(13):3207-15 - PubMed
    1. J Biol Chem. 1966 Jul 10;241(13):3216-21 - PubMed
    1. J Biol Chem. 1966 Jul 10;241(13):3222-7 - PubMed
    1. Ann N Y Acad Sci. 1966 Jun 30;133(2):315-33 - PubMed
    1. Nature. 1968 Mar 9;217(5132):955-7 - PubMed

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