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. 1973 Jan;70(1):200-4.
doi: 10.1073/pnas.70.1.200.

Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI

U Pettersson, C Mulder, H Deluis, P A Sharp

Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI

U Pettersson et al. Proc Natl Acad Sci U S A. 1973 Jan.

Abstract

The DNA of adenovirus type 2 was cleaved by restriction endonuclease R.RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 x 10(6) to 13.6 x 10(6), as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.

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