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. 1979 Mar;80(3):784-91.
doi: 10.1083/jcb.80.3.784.

Fibronectin from chicken embryo fibroblasts contains covalently bound phosphate

M H Teng, D B Rifkin

Fibronectin from chicken embryo fibroblasts contains covalently bound phosphate

M H Teng et al. J Cell Biol. 1979 Mar.

Abstract

Fibronectin isolated from cultures of chicken embryo fibroblasts (CEF) contains phosphorus linked to serine and threonine by monoester bonds. Normal and Rous sarcoma virus (RSV)-transformed cells were incubated with [32P]orthophosphate, and fibronectin was isolated from the cell surfaces and conditioned media. 32P was stably associated with fibronectin during immunoprecipitation, SDS-polyacrylamide gel electrophoresis, phospholipid solvent extraction, and hot acid but not alkaline treatment. After a limited acid hydrolysis of fibronectin, both phosphoserine and phosphothreonine were found. The specific radioactivity of the 32P-labeled fibronectin from the conditioned medium of normal CEF was higher than that from the cultures of transformed CEF.

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References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Nature. 1978 Sep 21;275(5677):179-84 - PubMed
    1. Cell. 1977 Aug;11(4):957-69 - PubMed
    1. Biochem Biophys Res Commun. 1977 Jan 10;74(1):300-7 - PubMed
    1. Hoppe Seylers Z Physiol Chem. 1977 Sep;358(9):1165-8 - PubMed

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