General method for the isolation of plasmid deoxyribonucleic acid
- PMID: 4583233
- PMCID: PMC285495
- DOI: 10.1128/jb.116.2.1064-1066.1973
General method for the isolation of plasmid deoxyribonucleic acid
Abstract
Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitation of the higher-molecular-weight chromosomal DNA in the presence of sodium lauryl sulfate and a high concentration of NaCl.
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